Loss of Retinol-Binding Properties for Plasma Retinol-Binding Protein in Normal Human Epidermis
نویسندگان
چکیده
منابع مشابه
Loss of retinol-binding properties for plasma retinol-binding protein in normal human epidermis.
Terminal differentiation of the keratinocytes (cornification) has been linked to a restricted supply of retinol. Retinol is distributed to target cells by the retinol-binding protein (RBP), which circulates in the plasma in complex with transthyretin (TTR). In this study we have addressed the question of retinol delivery to the epidermis via RBP. Retinol radiobinding assays, affinity chromatogr...
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A radioimmunoassay for human plasma retinol-binding protein (RBP) has been developed utilizing a double antibody precipitation technique. RBP was purified 1500- to 2000-fold by procedures described previously. A specific anti-human RBP antiserum was prepared in rabbits by three once-weekly injections of purified RBP emulsified with Freund's adjuvant. RBP was iodinated with (131)I and the RBP-(1...
متن کاملProperties of the chromophore binding site of retinol-binding protein from human plasma.
Apo-retinal-binding protein (apo-RBP) from human plasma was found to bind all-trans-3-dehydroretinol and to protect the chromophore from degradation. The all-trans-3dehydroretinol-RBP complex, in turn, binds tightly to prealbumin at physiological ionic strength as judged by gel filtration chromatography. All trans 3 dehydroretinal also formed a complex with RBP but this complex did not bind to ...
متن کاملThe interaction of human plasma retinol-binding protein and prealbumin.
The interaction of human plasma retinol-binding protein with plasma prealbumin was studied by the techniques of velocity ultracentrifugation and polarization of retinol fluorescence. In the first method the unbound fraction of retinol-binding protein, which sediments more slowly than its complexes with prealbumin, was measured by its absorption. A stoichiometry of retinol-binding protein to pre...
متن کاملRetinol-binding protein 4 in human obesity.
Studies in mice suggest that adipocytes serve as glucose sensors and regulate systemic glucose metabolism through release of serum retinol-binding protein 4 (RBP4). This model has not been validated in humans. RBP4 was highly expressed in isolated mature human adipocytes and secreted by differentiating human adipocytes. In contrast to the animal data, RBP4 mRNA was downregulated in subcutaneous...
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 1987
ISSN: 0022-202X
DOI: 10.1111/1523-1747.ep12469731